DIversité - Adaptation - DEveloppement des plantes


Senior researcher at CIRAD
PhD in Biology Molecular and Biochemistry at University of Montpellier.


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Current location: Veracruz/Xalapa, Mexico

Research interests

Dr J-C Breitler worked for 10 years in the rice adaptive development team as a molecular biologist in charge of functional genomics projects. In 2015, he joined the CoffeAdapt team as a molecular physiologist. In 2017, his "coffee Adapt team" in CIRAD obtained from UE, a H2020 project called "Breedcafs" to study the behavior of Hybrid F1 varieties of coffee against climate change and managed under agroforestry systems. An expatriate since 2017 in Mexico, he was responsible for monitoring the field trials of this project in Central America. After initiating work on plant health, he is currently developing projects involving regenerative agriculture and agroforestry in Mexico, with a special focus on soil health, plant health, productivity and coffee quality.

Ongoing fundings and projects

  • Las Canadas, Mexico - CIRAD - ECOM project (2023-2028):  Semi-industrial comparative study of 12 arabica varieties, including 4 F1 hybrids under agroforestry and regenerative agriculture conditions.
  • Belco, France - CIRAD - French Embassy in Mexico project (2023-2027):  Understanding the interrelationship between light and temperature on coffee flowering with the aim of creating new varieties better adapted to the shaded agroforestry systems (1 mexican PhD student)
  • Breeding4Futur (2023-2027):  Harnessing genetic diversity to increase productivity and quality. (collaboration CIRAD - ECOM)
  • PRCC Café Nord Laos (2023-2027):   (WP5 = Soil fertility in coffee plantations by organic means and impact on the health of coffee trees: laying the basis of regenerative agriculture concept on coffee)
  • CIRAD - Nestlé project (2021-2023):  Effects of drought (and other environmental parameters) in C. canephora: a proposed large scale approach toward GWAS and selection of new elite clones.
  • CIRAD - Nestlé project (2021-2023):  Identification of markers & candidate genes associated with male sterility in Coffea arabica.


  • Awada, R., Lepelley, M., Breton, D., Charpagne, A., Campa, C., Berry, V., Georget, F., Breitler, J.-C., Leran, S., Djerrab, D., Martinez-Seidel, F., Descombes, P., Crouzillat, D., Bertrand, B., & Etienne, H. (2023). Global transcriptome profiling reveals differential regulatory, metabolic and hormonal networks during somatic embryogenesis in #Coffea arabica#. BMC Genomics, 24, 19 p. https://doi.org/10.1186/s12864-022-09098-z

  • Mbebi, A. J., Breitler, J.-C., Bordeaux, M., Sulpice, R., McHale, M., Hao Tong, Toniutti, L., Alonso Castillo, J., Bertrand, B., & Nikoloski, Z. (2022). A comparative analysis of genomic and phenomic predictions of growth-related traits in three-way coffee hybrids. G3 - Genes Genomes Genetics, 12(9), 14 p. https://doi.org/10.1093/g3journal/jkac170

  • Casarin, T., Chagas Freitas, N., Terassi Pinto, R., Breitler, J.-C., Zebral Rodrigues, L. A., Marraccini, P., Etienne, H., Cardamone Diniz, L. E., Carvalho Andrade, A., & Vilela Paiva, L. (2022). Multiplex CRISPR/Cas9?mediated knockout of the #phytoene desaturase# gene in #Coffea canephora#. Scientific Reports, 12, 10 p. https://doi.org/10.1038/s41598-022-21566-w

  • Breitler, J.-C., Etienne, H., Leran, S., Marie, L., & Bertrand, B. (2022). Description of an Arabica coffee ideotype for agroforestry cropping systems : A guideline for breeding more resilient new varieties. Plants, 11(16), 24 p. https://doi.org/10.3390/plants11162133

  • Gamboa-Becerra, R., Lopez-Lima, D., Villain, L., Breitler, J.-C., Carrion, G., & Desgarennes, D. (2021). Molecular and environmental triggering factors of pathogenicity of #Fusarium oxysporum# and #F. solani# isolates involved in the coffee corky-root disease. Journal of Fungi, 7(4), 23 p. https://doi.org/10.3390/jof7040253

  • Djerrab, D., Bertrand, B., Breitler, J.-C., Leran, S., Dechamp, E., Campa, C., Barrachina, C., Conejero, G., Etienne, H., & Sulpice, R. (2021). Photoperiod-dependent transcriptional modifications in key metabolic pathways in #Coffea arabica#. Tree Physiology, 41(2), 302‑316. https://doi.org/10.1093/treephys/tpaa130

  • Bertrand, B., Villegas Hincapie, A. M., Marie, L., & Breitler, J.-C. (2021). Breeding for the main agricultural farming of Arabica coffee. Frontiers in Sustainable Food Systems, 5, 15 p. https://doi.org/10.3389/fsufs.2021.709901

  • Marie, L., Abdallah, C., Campa, C., Courtel, P., Bordeaux, M., Navarini, L., Lonzarich, V., Skovmand Bosselmann, A., Turreira-Garcia, N., Alpizar, E., Georget, F., Breitler, J.-C., Etienne, H., & Bertrand, B. (2020). G x E interactions on yield and quality in #Coffea arabica# : New F1 hybrids outperform American cultivars. Euphytica, 216, 17 p. https://doi.org/10.1007/s10681-020-02608-8

  • Duangsodsri, T., Villain, L., Vestalys, I. R., Michalet, S., Abdallah, C., Breitler, J.-C., Bordeaux, M., Villegas, A. M., Raherimandimby, M., Legendre, L., Etienne, H., Bertrand, B., & Campa, C. (2020). 5-CQA and mangiferin, two leaf biomarkers of adaptation to full sun or shade conditions in #Coffea arabica# L. Metabolites, 10(10), 22 p. https://doi.org/10.3390/metabo10100383

  • Breitler, J.-C., Djerrab, D., Leran, S., Toniutti, L., Guittin, C., Severac, D., Pratlong, M., Dereeper, A., Etienne, H., & Bertrand, B. (2020). Full moonlight-induced circadian clock entrainment in #Coffea arabica#. BMC Plant Biology, 20, 11 p. https://doi.org/10.1186/s12870-020-2238-4

  • Toniutti, L., Breitler, J.-C., Guittin, C., Doulbeau, S., Etienne, H., Campa, C., Lambot, C., Herrera Pinilla, J.-C., & Bertrand, B. (2019). An altered circadian clock coupled with a higher photosynthesis efficiency could explain the better agronomic performance of a new coffee clone when compared with a standard variety. International Journal of Molecular Sciences, 20(3), 19 p. https://doi.org/10.3390/ijms20030736

  • Georget, F., Marie, L., Alpizar, E., Courtel, P., Bordeaux, M., Hidalgo, M., Marraccini, P., Breitler, J.-C., Dechamp, E., Poncon, C., Etienne, H., & Bertrand, B. (2019). Starmaya : The first arabica F1 coffee hybrid produced using genetic male sterility. Frontiers in Plant Science, 10, 13 p. https://doi.org/10.3389/fpls.2019.01344

  • Ferreira Torres, L., Reichel, T., Dechamp, E., De Aquino Oliveira, S., Duarte, K. E., Costa Alves, G. S., Tadeu Silva, A., Guitton Cotta, M., Santos Costa, T., Cardamone Diniz, L. E., Breitler, J.-C., Collin, M., Vilela Paiva, L., Carvalho Andrade, A., Etienne, H., & Marraccini, P. (2019). Expression of #DREB#-like genes in #Coffea canephora# and #C. arabica# subjected to various types of abiotic stress. Tropical Plant Biology, 12(2), 98‑116. https://doi.org/10.1007/s12042-019-09223-5

  • Etienne, H., Breton, D., Breitler, J.-C., Bertrand, B., Dechamp, E., Awada, R., Marraccini, P., Leran, S., Alpizar, E., Campa, C., Courtel, P., Georget, F., & Ducos, J.-P. (2018). Coffee somatic embryogenesis : How did research, experience gained and innovations promote the commercial propagation of elite clones from the two cultivated species. Frontiers in Plant Science, 9, 21p. https://doi.org/10.3389/fpls.2018.01630

  • Breitler, J.-C., Dechamp, E., Campa, C., Rodrigues, L. A. Z., Guyot, R., Marraccini, P., & Etienne, H. (2018). CRISPR/Cas9-mediated efficient targeted mutagenesis has the potential to accelerate the domestication of #Coffea canephora#. Plant Cell, Tissue and Organ Culture, 134(3), 383‑394. https://doi.org/10.1007/s11240-018-1429-2

  • Toniutti, L., Breitler, J.-C., Etienne, H., Campa, C., Doulbeau, S., Urban, L., Lambot, C., Pinilla, J.-C. H., & Bertrand, B. (2017). Influence of environmental conditions and genetic background of #Arabica# coffee (#C. arabica L#) on leaf rust (#Hemileia vastatrix#)pathogenesis. Frontiers in Plant Science, 8, 12 p. https://doi.org/10.3389/fpls.2017.02025

  • Georget, F., Courtel, P., Garcia, E. M., Hidalgo, M., Alpizar, E., Breitler, J.-C., Bertrand, B., & Etienne, H. (2017). Somatic embryogenesis-derived coffee plantlets can be efficiently propagated by horticultural rooted mini-cuttings : A boost for somatic embryogenesis. Scientia Horticulturae, 216, 177‑185. https://doi.org/10.1016/j.scienta.2016.12.017

  • Costa Alves, G. S., Ferreira Torres, L., Dechamp, E., Breitler, J.-C., Joët, T., Gatineau, F., Carvalho Andrade, A., Bertrand, B., Marraccini, P., & Etienne, H. (2017). Differential fine-tuning of gene expression regulation in coffee leaves by #CcDREB1D# promoter haplotypes under water deficit. Journal of Experimental Botany, 68(11), 3017‑3031. https://doi.org/10.1093/jxb/erx166

  • Campa, C., Urban, L., Mondolot, L., Fabre, D., Roques, S., Lizzi, Y., Aarrouf, J., Doulbeau, S., Breitler, J.-C., Letrez, C., Toniutti, L., Bertrand, B., La Fisca, P., Bidel, L. P. R., & Etienne, H. (2017). Juvenile coffee leaves acclimated to low light are unable to cope with a moderate light increase. Frontiers in Plant Science, 8, 16 p. https://doi.org/10.3389/fpls.2017.01126

  • Etienne, H., Bertrand, B., Dechamp, E., Maurel, P., Georget, F., Guyot, R., & Breitler, J.-C. (2016). Are genetics and epigenetic instabilities of plant embryogenic cells a fatality ? The experience of coffee somatic embryogenesis. Human Genetics and Embryology, 6(136), 5 p. https://doi.org/10.4172/2161-0436.1000136

  • Breitler, J.-C., Campa, C., Georget, F., Bertrand, B., & Etienne, H. (2016). A single-step method for RNA isolation from tropical crops in the field. Scientific Reports, 6(38368), 6 p. https://doi.org/10.1038/srep38368

  • Pinto, L. M. N., Fiuza, L. M., Ziegler, D., De Oliveira, J. V., Menezes, V. G., Bourrié, I., Meynard, D., Guiderdoni, E., Breitler, J.-C., Altosaar, I., & Gantet, P. (2013). Indica rice cultivar IRGA 424, Ttansformed with cry genes of #B. thuringiensis#, provided High resistance against #Spodoptera frugiperda# (Lepidoptera : Noctuidae). Journal of Economic Entomology, 106(6), 2585‑2594. https://doi.org/10.1603/EC13163

  • Mieulet, D., Meynard, D., Sire, C., Petit, J., Breitler, J.-C., Perin, C., Diévart, A., Divol Malgoire, F., Courtois, B., Verdeil, J.-L., Gantet, P., & Guiderdoni, E. (2013). La transformation génétique, un outil pour l’analyse fonctionnelle du génome du riz. Cahiers Agricultures, 22(5), 484‑493. https://doi.org/10.1684/agr.2013.0650

  • Meynard, D., Mieulet, D., Ben Saad, R., Breitler, J.-C., Petit, J., Gantet, P., Hassairi, A., & Guiderdoni, E. (2013). Applications de la transgenèse à l’amélioration variétale du riz. Cahiers Agricultures, 22(5), 494‑505. https://doi.org/10.1684/agr.2013.0625

  • Andrieu, A., Breitler, J.-C., Sire, C., Meynard, D., Gantet, P., & Guiderdoni, E. (2012). An in planta, Agrobacterium-mediated transient gene expression method for inducing gene silencing in rice (#Oryza sativa# L.) leaves. Rice, 5(23), 12 p. https://doi.org/10.1186/1939-8433-5-23

  • Thomas, X., Hedhili, S., Beuf, L., Demattéi, M.-V., Laparra, H., Khong, N. G., Breitler, J.-C., Montandon, F., Carnus, E., Norre, F., Burtin, D., Gantet, P., Bigot, Y., & Renault, S. (2010). The mariner #Mos1# transposase produced in tobacco is active in vitro. Genetica, 138(5), 519‑530. https://doi.org/10.1007/s10709-009-9414-7

  • Shu-I Lin, Santi, C., Jobet, E., Lacut, E., El Kholti, N., Karlowski, W. M., Verdeil, J.-L., Breitler, J.-C., Perin, C., Swee-Suak Ko, Guiderdoni, E., Chiou, T. J., & Echeverria, M. (2010). Complex regulation of two target genes encoding SPX-MFS proteins by rice miR827 in response to phosphate starvation. Plant and Cell Physiology, 51(12), 2119‑2130. https://doi.org/10.1093/pcp/pcq170

  • Rebouillat, J., Dievart, A., Verdeil, J.-L., Escoute, J., Giese, G., Breitler, J.-C., Gantet, P., Espeout, S., Guiderdoni, E., & Perin, C. (2009). Molecular genetics of rice root development. Rice, 2(1), 15‑34. https://doi.org/10.1007/s12284-008-9016-5

  • Lacombe, S., Nagasaki, H., Santi, C., Duval, D., Piegu, B., Bangratz, M., Breitler, J.-C., Guiderdoni, E., Brugidou, C., Hirsch, J., Cao, X., Brice, C., Panaud, O., Karlowski, W. M., Sato, Y., & Echeverria, M. (2008). Identification of precursor transcripts for 6 novel miRNAs expands the diversity on the genomic organisation and expression of miRNA genes in rice. BMC Plant Biology, 8(123), 19 p. https://doi.org/10.1186/1471-2229-8-123

  • Guiderdoni, E., & Breitler, J.-C. (2006). Apport de la transgenèse à l’amélioration du riz. L’exemple du riz doré. Le Sélectionneur Français, 57, 57‑68. https://www.selectionneurs.asso.fr/le-bulletin/le-bulletin-2006/

  • Vila, L., Quilis, J., Meynard, D., Breitler, J.-C., Marfa, V., Murillo, I., Vassal, J.-M., Messeguer, J., Guiderdoni, E., & San Segundo, B. (2005). Expression of the maize proteinase inhibitor (mpi) gene in rice plants enhances resistance against the striped stem borer (#Chilo suppressalis#) : Effects on larval growth and insect gut proteinases. Plant Biotechnology Journal, 3(2), 187‑202. https://doi.org/10.1111/j.1467-7652.2004.00117.x

  • Breitler, J.-C., Vassal, J.-M., Del Mar Catala, M., Meynard, D., Marfa, V., Melé, E., Royer, M., Murillo, I., San Segundo, B., Guiderdoni, E., & Messeguer, J. (2004). #Bt# rice harbouring cry genes controlled by a constitutive or wound-inducible promoter : Protection and transgene expression under Mediterranean field conditions. Plant Biotechnology Journal, 2(5), 417‑430. https://doi.org/10.1111/j.1467-7652.2004.00086.x

  • Breitler, J.-C., Meynard, D., Van Boxtel, J., Royer, M., Bonnot, F., Cambillau, L., & Guiderdoni, E. (2004). A novel two T-DNA binary vector allows efficient generation of marker-free transgenic plants in three elite cultivars of rice (#Oryza sativa# L.). Transgenic Research, 13(3), 271‑287. https://doi.org/10.1023/B:TRAG.0000034626.22918.0a

  • Breitler, J.-C. (2003). OGM, ces plantes qui dérangent. Pour, 178, 136‑140.

  • Cotsaftis, O., Sallaud, C., Breitler, J.-C., Meynard, D., Greco, R., Pereira, A., & Guiderdoni, E. (2002). Transposon-mediated generation of T-DNA - and marker-free rice plants expressing a #Bt# endotoxin gene. Molecular Breeding, 10, 165‑180. https://doi.org/10.1023/A:1020380305904

  • Breitler, J.-C., Labeyrie, A., Meynard, D., Legavre, T., & Guiderdoni, E. (2002). Efficient microprojectile bombardment-mediated transformation of rice using gene cassettes. Theoretical and Applied Genetics, 104(4), 709‑719. https://doi.org/10.1007/s00122-001-0786-z

  • Breitler, J.-C., Cordero, M. J., Royer, M., Meynard, D., San Segundo, B., & Guiderdoni, E. (2001). The -689/+197 region of the maize protease inhibitor gene directs high level, wound-inducible expression of the #cry1B# gene which protects transgenic rice plants from stemborer attack. Molecular Breeding, 7, 259‑274.  https://doi.org/10.1023/A:1011609128319

  • Breitler, J.-C., Marfa, V., Royer, M., Meynard, D., Vassal, J.-M., Vercambre, B., Frutos, R., Messeguer, J., Gabarra, R., & Guiderdoni, E. (2000). Expression of a #Bacillus thuringiensis# #cry1B# synthetic gene protects Mediterranean rice against the striped stem borer. Plant Cell Reports, 19, 1195‑1202. https://doi.org/10.1007/s002990000247

  • Baurens, F.-C., Breitler, J.-C., & Lagoda, P. (1999). Une transformation génétique des bananes et plantains écologiquement acceptable ? Proposition pour une expérimentation théorique, non limitée au seul genre #Musa# (Vitropic - FRA; CIRAD-AMIS - FRA, Trad.). Fruits, 54(5), 371‑384. https://revues.cirad.fr/index.php/fruits

  • Cellier, F., Conejero, G., Breitler, J.-C., & Casse, F. (1998). Molecular and physiological responses to water deficit in drought-tolerant and drought-sensitive lines of sunflower. Accumulation of dehydrin transcripts correlates with tolerance. Plant Physiology, 116, 319‑328. https://doi.org/10.1104/pp.116.1.319

Books and chapters

  • Meynard, D., Vernet, A., Meunier, A. C., Mieulet, D., Bes, M., Portefaix, M., Breitler, J.-C., Périn, C., & Guiderdoni, E. (2020). Thirty years of genome engineering in rice : From gene addition to gene editing. In Roberts Jeremy (ed.) (Éd.), Annual plant reviews online (Agritrop : 595666; 1‑3, p. 1‑76). John Wiley & Sons; https://doi.org/10.1002/9781119312994.apr0662

  • Bertrand, B., Breitler, J.-C., Georget, F., Penot, E., Bordeaux, M., Marraccini, P., Leran, S., Campa, C., Bonato, O., Villain, L., & Etienne, H. (2019). New varieties for innovative agroforestry coffee systems. In P.-M. E. (ed. ) Côte François-Xavier (ed.) Perret Sylvain (ed. ), Roudier Philippe (ed. ), Bruno Rapidel (ed. ), Thirion Marie-Cécile (ed. ). (Éd.), The agroecological transition of agricultural systems in the Global South (Agritrop : 592997; p. 161‑176). Ed. Quae; https://www.quae-open.com/produit/114/9782759230570/the-agroecological-transition-of-agricultural-systems-in-the-global-south

  • Bertrand, B., Breitler, J.-C., Georget, F., Penot, E., Bordeaux, M., Marraccini, P., Leran, S., Campa, C., Bonato, O., Villain, L., & Etienne, H. (2018). De nouvelles variétés pour des systèmes caféiers agroforestiers innovants. In P.-M. E. (ed. ) Côte François-Xavier (ed.) Perret Sylvain (ed. ), Roudier Philippe (ed. ), Bruno Rapidel (ed. ), Thirion Marie-Cécile (ed. ). (Éd.), La transition agro-écologique des agricultures du Sud (Agritrop : 590491; p. 231‑256). Ed. Quae; https://www.quae.com/produit/1546/9782759228232/la-transition-agro-ecologique-des-agricultures-du-sud

  • Etienne, H., Guyot, R., Beulé, T., Breitler, J.-C., & Jaligot, E. (2016). Plant fidelity in somatic embryogenesis-regenerated plants. In O.-A. N. (ed. ) M Loyola-Vargas Victor (ed.) (Éd.), Somatic embryogenesis : Fundamental aspects and applications (Agritrop : 581502; p. 121‑150). Springer International Publishing; https://doi.org/10.1007/978-3-319-33705-0_8

  • Bertrand, B., Marraccini, P., Villain, L., Breitler, J.-C., & Etienne, H. (2016). Healthy tropical plants to mitigate the impact of climate change—As exemplified in coffee. In M. D. (trad. ) Torquebiau Emmanuel (ed.) Cowan Paul (trad. ). (Éd.), Climate change and agriculture worldwide (Agritrop : 578422; p. 83‑95). Springer; https://doi.org/10.1007/978-94-017-7462-8_7

  • Dechamp, E., Breitler, J.-C., Leroy, T., & Etienne, H. (2015). Coffee (#Coffea arabica L.#). In Wang Kan  (ed.) (Éd.), Agrobacterium Protocols : Volume 2 (Agritrop : 577283; 3rd ed, 1‑1224, p. 275‑291). Springer; https://doi.org/10.1007/978-1-4939-1658-0_22

  • Bertrand, B., Marraccini, P., Villain, L., Breitler, J.-C., & Etienne, H. (2015). La bonne santé des plantes tropicales pour atténuer les effets du changement climatique : L’exemple du caféier. In Torquebiau Emmanuel (ed.) (Éd.), Changement climatique et agricultures du monde (Agritrop : 575564; p. 84‑95). Ed. Quae

  • Khong, N. G., Richaud, F., Coudert, Y., Pati, P. K., Santi, C., Perin, C., Breitler, J.-C., Meynard, D., Guiderdoni, E., & Gantet, P. (2008). Modulating rice stress tolerance by transcription factors. In Ed. by Stephen E. Harding (Éd.), Biotechnology and genetic engineering reviews. Vol. 25 (Agritrop : 548135; p. 381‑404). Nottingham University Press

  • Breitler, J.-C. (2002). Les biotechnologies végétales : Historique, définition et application au riz. In Mémento de l’agronome (Agritrop : 511912; p. 1 Cd-Rom). CIRAD

  • Casse, F., & Breitler, J.-C. (2001). OGM. Description, méthodes d’obtention, domaines d’application (Agritrop : 484748). France agricole


Research of the COFFEADAPT team
COFFEEADAPT team publication
Team members
Permanent and temporary staff