DIversité - Adaptation - DEveloppement des plantes


Senior research at CIRAD
PhD in Cell Biology at Paris VI University.


Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Current location: IRD, Campus Lavalette, Montpellier, France

Research interests

Dr Hervé Etienne has over 32 years of experience in Cell biology and biotechnologies. Actually, working as deputy director of the research unit DIADE. His research activities are focused on functional genomics of coffee and in biotechnologies (micropropagation, somatic embryogenesis). In the frame of the ECOM-CIRAD alliance, he is involved in the dissemination strategy of Arabica hybrid varieties throughout the world but mainly in Latin America, Central Africa and South East Asia, and of the monitoring of agronomic performance. He has co-coordinated the European project H2020 BREEDCAFS (2017-2022) and currently participate in the European projects Desira ROBUST (2021-2025) and Horizon Europe BOLERO (2022-2026) through research activities on the functional genomics of genes involved in root architecture and the technology transfer of vegetative propagation techniques for elite coffee varieties in vitro and in horticulture. He also initiates pioneer works in the development of Temporary Immersion Bioreactors in plant micropropagation.

Ongoing fundings and projects


  • Sarzynski, T., Bertrand, B., Rigal, C., Marraccini, P., Vaast, P., Georget, F., Campa, C., Abdallah, C., Nguyen, T. Q. C., Nguyen, H. P., Nguyen, H. T. T., Ngoc, Q. L., Ngan, G. K., Viet, T. V., Navarini, L., Lonzarich, V., Bossolasco, L., & Etienne, H. (2023). Genetic-environment interactions and climatic variables effect on bean physical characteristics and chemical composition of #Coffea arabica#. Journal of the Science of Food and Agriculture, 103, 4692‑4703. https://doi.org/10.1002/jsfa.12544

  • Meter, A., Penot, E., Vaast, P., Etienne, H., Ponçon, E., & Bertrand, B. (2023). Local value-chains dedicated to sustainable production (coffee agroforestry business-driven clusters or CaFC) : A new organizational model to foster social and environmental innovations through farm renovation [version 2; peer review : 2 approved]. Open Research Europe, 2, 38 p. https://doi.org/10.12688/openreseurope.14570.2

  • Koutouleas, A., Blunt, C., Bregar, A., Hansen, J. K., Ræbild, A., Etienne, H., & Georget, F. (2023). Effects of interspecific grafting of #Coffea arabica# and elevation on coffee growth, yield, and quality attributes in Costa Rica. Scientia Horticulturae, 320, 16 p. https://doi.org/10.1016/j.scienta.2023.112162

  • Awada, R., Lepelley, M., Breton, D., Charpagne, A., Campa, C., Berry, V., Georget, F., Breitler, J.-C., Leran, S., Djerrab, D., Martinez-Seidel, F., Descombes, P., Crouzillat, D., Bertrand, B., & Etienne, H. (2023). Global transcriptome profiling reveals differential regulatory, metabolic and hormonal networks during somatic embryogenesis in #Coffea arabica#. BMC Genomics, 24, 19 p. https://doi.org/10.1186/s12864-022-09098-z

  • Koutouleas, A., Sarzynski, T., Bordeaux, M., Skovmand Bosselmann, A., Campa, C., Etienne, H., Turreira-Garcia, N., Rigal, C., Vaast, P., Cochicho Ramalho, J., Marraccini, P., & Raebild, A. (2022). Shaded-coffee : A nature-based strategy for coffee production under climate change ? A review. Frontiers in Sustainable Food Systems, 6, 21 p. https://doi.org/10.3389/fsufs.2022.877476

  • Koutouleas, A., Sarzynski, T., Bertrand, B., Bordeaux, M., Skovmand Bosselmann, A., Campa, C., Etienne, H., Turreira-Garcia, N., Leran, S., Markussen, B., Marraccini, P., Cochicho Ramalho, J., Vaast, P., & Raebild, A. (2022). Shade effects on yield across different #Coffea arabica cultivars# ? How much is too much ? A meta-analysis. Agronomy for Sustainable Development, 42, 13 p. https://doi.org/10.1007/s13593-022-00788-2

  • Duong, B., Marraccini, P., Etienne, H., Villain, L., Hoang, T. G., Khong, N. G., Lebrun, M., & Duponnois, R. (2022). Potential of the coffee endophytic #Bacillus cereus sensu lato# strain CCBLR15 to control the plant-parasitic nematode #Radopholus duriophilus#. Biocontrol Science and Technology, 32(8), 971‑988. https://doi.org/10.1080/09583157.2022.2070600

  • de Lourdes Tapia y Figueroa, M., Beraún Tapia, J. F., Hajari, E., Escalona, M., Etienne, H., & Lorenzo, J. C. (2022). Agronomic performances of temporary immersion bioreactor-derived potato microtubers in a Peruvian low input cropping agriculture system. African Journal of Biotechnology, 21(3), 125‑132. https://doi.org/10.5897/AJB2021.17430

  • Casarin, T., Chagas Freitas, N., Terassi Pinto, R., Breitler, J.-C., Zebral Rodrigues, L. A., Marraccini, P., Etienne, H., Cardamone Diniz, L. E., Carvalho Andrade, A., & Vilela Paiva, L. (2022). Multiplex CRISPR/Cas9?mediated knockout of the #phytoene desaturase# gene in #Coffea canephora#. Scientific Reports, 12, 10 p. https://doi.org/10.1038/s41598-022-21566-w

  • Breitler, J.-C., Etienne, H., Leran, S., Marie, L., & Bertrand, B. (2022). Description of an Arabica coffee ideotype for agroforestry cropping systems : A guideline for breeding more resilient new varieties. Plants, 11(16), 24 p. https://doi.org/10.3390/plants11162133

  • Djerrab, D., Bertrand, B., Breitler, J.-C., Leran, S., Dechamp, E., Campa, C., Barrachina, C., Conejero, G., Etienne, H., & Sulpice, R. (2021). Photoperiod-dependent transcriptional modifications in key metabolic pathways in #Coffea arabica#. Tree Physiology, 41(2), 302‑316. https://doi.org/10.1093/treephys/tpaa130

  • Marie, L., Abdallah, C., Campa, C., Courtel, P., Bordeaux, M., Navarini, L., Lonzarich, V., Skovmand Bosselmann, A., Turreira-Garcia, N., Alpizar, E., Georget, F., Breitler, J.-C., Etienne, H., & Bertrand, B. (2020). G x E interactions on yield and quality in #Coffea arabica# : New F1 hybrids outperform American cultivars. Euphytica, 216, 17 p. https://doi.org/10.1007/s10681-020-02608-8

  • Leran, S., Noguero, M., Corratgé-Faillie, C., Boursiac, Y., Brachet, C., & Lacombe, B. (2020). Functional characterization of the arabidopsis abscisic acid transporters NPF4.5 and NPF4.6 in xenopus oocytes. Frontiers in Plant Science, 11, 6 p. https://doi.org/10.3389/fpls.2020.00144

  • Duangsodsri, T., Villain, L., Vestalys, I. R., Michalet, S., Abdallah, C., Breitler, J.-C., Bordeaux, M., Villegas, A. M., Raherimandimby, M., Legendre, L., Etienne, H., Bertrand, B., & Campa, C. (2020). 5-CQA and mangiferin, two leaf biomarkers of adaptation to full sun or shade conditions in #Coffea arabica# L. Metabolites, 10(10), 22 p. https://doi.org/10.3390/metabo10100383

  • Breitler, J.-C., Djerrab, D., Leran, S., Toniutti, L., Guittin, C., Severac, D., Pratlong, M., Dereeper, A., Etienne, H., & Bertrand, B. (2020). Full moonlight-induced circadian clock entrainment in #Coffea arabica#. BMC Plant Biology, 20, 11 p. https://doi.org/10.1186/s12870-020-2238-4

  • Awada, R., Verdier, D., Froger, S., Brulard, E., de Faria Maraschin, S., Etienne, H., & Breton, D. (2020). An innovative automated active compound screening system allows high-throughput optimization of somatic embryogenesis in #Coffea arabica#. Scientific Reports, 10, 14 p. https://doi.org/10.1038/s41598-020-57800-6

  • Toniutti, L., Breitler, J.-C., Guittin, C., Doulbeau, S., Etienne, H., Campa, C., Lambot, C., Herrera Pinilla, J.-C., & Bertrand, B. (2019). An altered circadian clock coupled with a higher photosynthesis efficiency could explain the better agronomic performance of a new coffee clone when compared with a standard variety. International Journal of Molecular Sciences, 20(3), 19 p. https://doi.org/10.3390/ijms20030736

  • Georget, F., Marie, L., Alpizar, E., Courtel, P., Bordeaux, M., Hidalgo, M., Marraccini, P., Breitler, J.-C., Dechamp, E., Poncon, C., Etienne, H., & Bertrand, B. (2019). Starmaya : The first arabica F1 coffee hybrid produced using genetic male sterility. Frontiers in Plant Science, 10, 13 p. https://doi.org/10.3389/fpls.2019.01344

  • Ferreira Torres, L., Reichel, T., Dechamp, E., De Aquino Oliveira, S., Duarte, K. E., Costa Alves, G. S., Tadeu Silva, A., Guitton Cotta, M., Santos Costa, T., Cardamone Diniz, L. E., Breitler, J.-C., Collin, M., Vilela Paiva, L., Carvalho Andrade, A., Etienne, H., & Marraccini, P. (2019). Expression of #DREB#-like genes in #Coffea canephora# and #C. arabica# subjected to various types of abiotic stress. Tropical Plant Biology, 12(2), 98‑116. https://doi.org/10.1007/s12042-019-09223-5

  • Awada, R., Campa, C., Gibault, E., Dechamp, E., Georget, F., Lepelley, M., Abdallah, C., Erban, A., Martinez-Seidel, F., Kopka, J., Legendre, L., Leran, S., Conejero, G., Verdeil, J.-L., Crouzillat, D., Breton, D., Bertrand, B., & Etienne, H. (2019). Unravelling the metabolic and hormonal machinery during key steps of somatic embryogenesis : A case study in coffee. International Journal of Molecular Sciences, 20(19), 29 p. https://doi.org/10.3390/ijms20194665

  • Varala, K., Marshall-Colón, A., Cirrone, J., Brooks, M. D., Pasquino, A. V., Leran, S., Mittal, S., Rock, T. M., Edwards, M. B., Kim, G. J., Ruffel, S., McCombie, R., Shasha, D., & Coruzzi, G. M. (2018). Temporal transcriptional logic of dynamic regulatory networks underlying nitrogen signaling and use in plants. Proceedings of the National Academy of Sciences of the United States of America, 115(25), 6494‑6499. https://doi.org/10.1073/pnas.1721487115

  • Hermoso Gallardo, L., Suárez-Villasmil, L., Etienne, H., Bertrand, B., Barry-Etienne, D., & Menendez-Yuffa, A. (2018). Semejanza entre el crecimiento en vivero de cafetos (Coffea arabica L.) obtenidos por embriogénesis somática y por semillas. Revista Biodiversidad Neotropical, 8(3), 11 p. https://doi.org/10.18636/bioneotropical.v8i2.356

  • Etienne, H., Breton, D., Breitler, J.-C., Bertrand, B., Dechamp, E., Awada, R., Marraccini, P., Leran, S., Alpizar, E., Campa, C., Courtel, P., Georget, F., & Ducos, J.-P. (2018). Coffee somatic embryogenesis : How did research, experience gained and innovations promote the commercial propagation of elite clones from the two cultivated species. Frontiers in Plant Science, 9, 21p. https://doi.org/10.3389/fpls.2018.01630

  • Dussert, S., Serret, J., Bastos-Siqueira, A., Morcillo, F., Dechamp, E., Rofidal, V., Lashermes, P., Etienne, H., & Joët, T. (2018). Integrative analysis of the late maturation programme and desiccation tolerance mechanisms in intermediate coffee seeds. Journal of Experimental Botany, 69(7), 1583‑1597. https://doi.org/10.1093/jxb/erx492

  • Costa Alves, G. S., Ferreira Torres, L., De Aquino Oliveira, S., Reichel, T., Freire, L. P., Gomes Vieira, N., Vinecky, F., This, D., Pot, D., Etienne, H., Vilela Paiva, L., Marraccini, P., & Carvalho Andrade, A. (2018). Nucleotide diversity of the coding and promoter rRegions of #DREB1D#, a candidate gene for drought tolerance in #Coffea Species#. Tropical Plant Biology, 11(1‑2), 31‑48. https://doi.org/10.1007/s12042-018-9199-x

  • Breitler, J.-C., Dechamp, E., Campa, C., Rodrigues, L. A. Z., Guyot, R., Marraccini, P., & Etienne, H. (2018). CRISPR/Cas9-mediated efficient targeted mutagenesis has the potential to accelerate the domestication of #Coffea canephora#. Plant Cell, Tissue and Organ Culture, 134(3), 383‑394. https://doi.org/10.1007/s11240-018-1429-2

  • Totev Valkov, V., Rogato, A., Martins Alves, L., Sol, S., Noguero, M., Leran, S., Lacombe, B., & Chiurazzi, M. (2017). The nitrate transporter family protein LjNPF8.6 controls the N-fixing nodule activity. Plant Physiology, 175, 1269‑1282. https://doi.org/10.1104/pp.17.01187

  • Toniutti, L., Breitler, J.-C., Etienne, H., Campa, C., Doulbeau, S., Urban, L., Lambot, C., Pinilla, J.-C. H., & Bertrand, B. (2017). Influence of environmental conditions and genetic background of #Arabica# coffee (#C. arabica L#) on leaf rust (#Hemileia vastatrix#)pathogenesis. Frontiers in Plant Science, 8, 12 p. https://doi.org/10.3389/fpls.2017.02025

  • Georget, F., Courtel, P., Garcia, E. M., Hidalgo, M., Alpizar, E., Breitler, J.-C., Bertrand, B., & Etienne, H. (2017). Somatic embryogenesis-derived coffee plantlets can be efficiently propagated by horticultural rooted mini-cuttings : A boost for somatic embryogenesis. Scientia Horticulturae, 216, 177‑185. https://doi.org/10.1016/j.scienta.2016.12.017

  • Costa Alves, G. S., Ferreira Torres, L., Dechamp, E., Breitler, J.-C., Joët, T., Gatineau, F., Carvalho Andrade, A., Bertrand, B., Marraccini, P., & Etienne, H. (2017). Differential fine-tuning of gene expression regulation in coffee leaves by #CcDREB1D# promoter haplotypes under water deficit. Journal of Experimental Botany, 68(11), 3017‑3031. https://doi.org/10.1093/jxb/erx166

  • Campa, C., Urban, L., Mondolot, L., Fabre, D., Roques, S., Lizzi, Y., Aarrouf, J., Doulbeau, S., Breitler, J.-C., Letrez, C., Toniutti, L., Bertrand, B., La Fisca, P., Bidel, L. P. R., & Etienne, H. (2017). Juvenile coffee leaves acclimated to low light are unable to cope with a moderate light increase. Frontiers in Plant Science, 8, 16 p. https://doi.org/10.3389/fpls.2017.01126

  • Etienne, H., Bertrand, B., Dechamp, E., Maurel, P., Georget, F., Guyot, R., & Breitler, J.-C. (2016). Are genetics and epigenetic instabilities of plant embryogenic cells a fatality ? The experience of coffee somatic embryogenesis. Human Genetics and Embryology, 6(136), 5 p. https://doi.org/10.4172/2161-0436.1000136

  • Breitler, J.-C., Campa, C., Georget, F., Bertrand, B., & Etienne, H. (2016). A single-step method for RNA isolation from tropical crops in the field. Scientific Reports, 6(38368), 6 p. https://doi.org/10.1038/srep38368

  • Bouguyon, E., Brun, F., Meynard, D., Kubes, M., Pervent, M., Leran, S., Lacombe, B., Krouk, G., Guiderdoni, E., Zazimalova, E., Hoyerova, K., Nacry, P., & Gojon, A. (2015). Multiple mechanisms of nitrate sensing by #Arabidopsis# nitrate transceptor NRT1.1. Nature Plants, 1(3), 8 p. https://doi.org/10.1038/nplants.2015.15

  • Bobadilla Landey, R., Cenci, A., Guyot, R., Bertrand, B., Georget, F., Dechamp, E., Herrera, J. C., Aribi, J., Lashermes, P., & Etienne, H. (2015). Assessment of genetic and epigenetic changes during cell culture ageing and relations with somaclonal variation in #Coffea arabica#. Plant Cell, Tissue and Organ Culture, 122(3), 517‑531. https://doi.org/10.1007/s11240-015-0772-9

  • Bertrand, B., Bardil, A., Baraille, H., Dussert, S., Doulbeau, S., Dubois, E., Severac, D., Dereeper, A., & Etienne, H. (2015). The greater phenotypic homeostasis of the allopolyploid #Coffea arabica# improved the transcriptional homeostasis over that of both diploid parents. Plant and Cell Physiology, 56(10), 2035‑2051. https://doi.org/10.1093/pcp/pcv117

  • Polzin, F., Sylvestre, I., Dechamp, E., Ilbert, P., Etienne, H., & Engelmann, F. (2014). Effect of activated charcoal on multiplication of African yam (#Dioscorea cayenensis-rotundata#) nodal segments using a temporary immersion bioreactor (RITA(R)). In Vitro Cellular and Developmental Biology. Plant, 50(2), 210‑216. https://doi.org/10.1007/s11627-013-9552-6

  • Petitot, A.-S., Barsalobres-Cavallari, C., Ramiro, D., Albuquerque, E., Etienne, H., & Fernandez, D. (2013). Promoter analysis of the WRKY transcription factors #CaWRKY1a# and #CaWRKY1b# homoeologous genes in coffee (#Coffea arabica#). Plant Cell Reports, 32(8), 1263‑1276. https://doi.org/10.1007/s00299-013-1440-3

  • Etienne, H., Bertrand, B., Georget, F., Lartaud, M., Montes, F., Dechamp, E., Verdeil, J.-L., & Barry-Etienne, D. (2013). Development of coffee somatic and zygotic embryos to plants differs in the morphological, histochemical and hydration aspects. Tree Physiology, 33(6), 640‑653. https://doi.org/10.1093/treephys/tpt034

  • Bodadilla Landey, R., Cenci, A., Georget, F., Bertrand, B., Camayo, G., Dechamp, E., Herrera, J. C., Santoni, S., Lashermes, P., Simpson, J., & Etienne, H. (2013). High genetic and epigenetic stability in coffea arabica plants derived from embryogenic suspensions and secondary embryogenesis as revealed by AFLP, MSAP and the phenotypic variation rate. PloS One, 8(2), 15 p. https://doi.org/10.1371/journal.pone.0056372

  • Etienne, H., Bertrand, B., Montagnon, C., Bodadilla Landey, R., Dechamp, E., Jourdan, I., Alpizar, E., Malo, E., & Georget, F. (2012). Un exemple de transfert de technologie réussi dans le domaine de la micropropagation : La multiplication de #Coffea arabica# par embryogenèse somatique. Cahiers Agricultures, 21(2‑3), 115‑124. https://doi.org/10.1684/agr.2012.0553

  • Bertrand, B., Montagnon, C., Georget, F., Charmetant, P., & Etienne, H. (2012). Création et diffusion de variétés de caféiers Arabica : Quelles innovations variétales ? Cahiers Agricultures, 21(2‑3), 77‑88. https://doi.org/10.1684/agr.2012.0547

  • Ribas, A., Dechamp, E., Champion, A., Bertrand, B., Combes, M.-C., Verdeil, J.-L., Lapeyre, F., Lashermes, P., & Etienne, H. (2011). Agrobacterium-mediated genetic transformation of #Coffea arabica# (L.) is greatly enhanced by using established embryogenic callus cultures. BMC Plant Biology, 11(92), 15 p. https://doi.org/10.1186/1471-2229-11-92

  • Ribas, A., Cenci, A., Combes, M.-C., Etienne, H., & Lashermes, P. (2011). Organization and molecular evolution of a disease-resistance gene cluster in coffee trees. BMC Genomics, 12(240), 12 p. https://doi.org/10.1186/1471-2164-12-240

  • Bertrand, B., Alpizar, E., Lara, L., Santacreo, R., Hidalgo, M., Quijano, J. M., Montagnon, C., Georget, F., & Etienne, H. (2011). Performance of #Coffea arabica# F1 hybrids in agroforestry and full-sun cropping systems in comparison with American pure line cultivars. Euphytica, 181(2), 147‑158. https://doi.org/10.1007/s10681-011-0372-7

  • Menendez-Yuffa, A., Barry-Etienne, D., Bertrand, B., Georget, F., & Etienne, H. (2010). A comparative analysis of the development and quality of nursery plants derived from somatic embryogenesis and from seedlings for large-scale propagation of coffee (#Coffea arabica# L.). Plant Cell, Tissue and Organ Culture, 102(3), 297‑307. https://doi.org/10.1007/s11240-010-9734-4

  • Lashermes, P., Combes, M.-C., Ribas, A., Cenci, A., Mahé, L., & Etienne, H. (2010). Genetic and physical mapping of the SH3 region that confers resistance to leaf rust in coffee tree (#Coffea arabica# L.). Tree Genetics and Genomes, 6(6), 973‑980. https://doi.org/10.1007/s11295-010-0306-x

  • Alpizar, E., Dechamp, E., Lapeyre-Montes, F., Guilhaumon, C., Bertrand, B., Jourdan, C., Lashermes, P., & Etienne, H. (2008). #Agrobacterium rhizogenes#-transformed roots of Coffee (#Coffea arabica#) : Conditions for long-term proliferation, and morphological and molecular characterization. Annals of Botany, 101(7), 929‑940. https://doi.org/10.1093/aob/mcn027

  • Alpizar, E., Etienne, H., & Bertrand, B. (2007). Intermediate resistance to #Meloidogyne exigua# root-knot nematode in #Coffea arabica#. Crop Protection, 26(7), 903‑910. https://doi.org/10.1016/j.cropro.2006.08.018

  • Etienne, H., Dechamp, E., Barry-Etienne, D., & Bertrand, B. (2006). Bioreactors in coffee micropropagation. Brazilian Journal of Plant Physiology, 18(1), 45‑54.

  • Dussert, S., Davey, M. W., Laffargue, A., Doulbeau, S., Swennen, R., & Etienne, H. (2006). Oxidative stress, phospholipid loss and lipid hydrolysis during drying and storage of termediate seeds. Physiologia Plantarum, 127(2), 192‑204. https://doi.org/10.1111/j.1399-3054.2006.00666.x

  • Delannoy, E., Marmey, P., Jalloul, A., Etienne, H., & Nicole, M. (2006). Molecular biology and physiology : Molecular analysis of class III peroxidases from cotton. Journal of Cotton Science, 10(1), 53‑60.

  • Bertrand, B., Vaast, P., Alpizar, E., Etienne, H., Davrieux, F., & Charmetant, P. (2006). Comparison of bean biochemical composition and beverage quality of arabica hybrids involving Sudanese-Ethiopian origins with traditional varieties at various elevations in Central America. Tree Physiology, 26(9), 1239‑1248. https://doi.org/10.1093/treephys/26.9.1239

  • Alpizar, E., Dechamp, E., Espeout, S., Royer, M., Lecouls, A.-C., Nicole, M., Bertrand, B., Lashermes, P., & Etienne, H. (2006). Efficient production of #Agrobacterium rhizogenes#-transformed roots and composite plants for studying gene expression in coffee roots. Plant Cell Reports, 25, 959‑967. https://doi.org/10.1007/s00299-006-0159-9

  • Bertrand, B., Etienne, H., Lashermes, P., Guyot, B., & Davrieux, F. (2005). Can near-infrared reflectance of green coffee be used to detect introgression in #Coffea arabica# cultivars? Journal of the Science of Food and Agriculture, 85(6), 955‑962. https://doi.org/10.1002/jsfa.2049

  • Bertrand, B., Etienne, H., Cilas, C., Charrier, A., & Baradat, P. (2005). #Coffea arabica# hybrid performance for yield, fertility and bean weight. Euphytica, 141(3), 255‑262. https://doi.org/10.1007/s10681-005-7681-7

  • Albarran, J., Bertrand, B., Lartaud, M., & Etienne, H. (2005). Cycle characteristics in a temporary immersion bioreactor affect regeneration, morphology, water and mineral status of coffee (#Coffea arabica#) somatic embryos. Plant Cell, Tissue and Organ Culture, 81, 27‑36. https://doi.org/10.1007/s11240-004-2618-8

  • Etienne, H., & Bertrand, B. (2003). Somaclonal variation in #Coffea arabica# : Effects of genotype and embryogenic cell suspension age on frequency and phenotype of variants. Tree Physiology, 23(6), 419‑426. https://doi.org/10.1093/treephys/23.6.419

  • Etienne, H., & Berthouly, M. (2002). Temporary immersion systems in plant micropropagation. Plant Cell, Tissue and Organ Culture, 69(3), 215‑231. https://doi.org/10.1023/A:1015668610465

  • Etienne, H., Anthony, F., Dussert, S., Fernandez, D., Lashermes, P., & Bertrand, B. (2002). Biotechnological applications for the improvement of coffee (#Coffea arabica# L.). In Vitro Cellular and Developmental Biology. Plant, 38(2), 129‑138. https://doi.org/10.1079/IVP2001273

  • Barry-Etienne, D., Bertrand, B., Vasquez, N., & Etienne, H. (2002). Comparison of somatic embryogenesis-derived coffee (#Coffea arabica# L.) plantlets regenerated #in vitro# or #ex vitro# : Morphological, mineral and water characteristics. Annals of Botany, 90(1), 77‑85. https://doi.org/10.1093/aob/mcf149

  • Barry-Etienne, D., Bertrand, B., Schlönvoigt, A., & Etienne, H. (2002). The morphological variability within a population of coffee somatic embryos produced in a bioreactor affects the regeneration and the development of plants in the nursery. Plant Cell, Tissue and Organ Culture, 68, 153‑162. https://doi.org/10.1023/A:1013874221569

  • Etienne, H., & Bertrand, B. (2001). Trueness-to-type and agronomic characteristics of #Coffea arabica# trees micropropagated by the embryogenic cell suspension technique. Tree Physiology, 21(14), 1031‑1038. https://doi.org/10.1093/treephys/21.14.1031

  • Bertrand, B., Etienne, H., & Eskes, A. (2001). Growth, production, and bean quality of #Coffea arabica# as affected by interspecific grafting : Consequences for rootstock breeding. HortScience, 36(2), 269‑273. https://doi.org/10.21273/HORTSCI.36.2.269

  • Bertrand, B., Pena Duran, M. X., Anzueto, F., Cilas, C., Etienne, H., Anthony, F., & Eskes, A. (2000). Genetic study of #Coffea canephora# coffee tree resistance to #Meloidogyne incognita# nematodes in Guatemala and #Meloidogyne# sp. Nematodes in El Salvador for selection of rootstock varieties in Central America. Euphytica, 113(2), 79‑86. https://doi.org/10.1023/A:1003931918187

  • Etienne Barry, D., Bertrand, B., Vasquez, N., & Etienne, H. (1999). Direct sowing of #Coffea arabica# somatic embryos mass-produced in a bioreactor and regeneration of plants. Plant Cell Reports, 19(2), 111‑117. https://doi.org/10.1007/s002990050720

  • Etienne, H., Solano, W., Pereira, A., Barry-Etienne, D., Bertrand, B., Anthony, F., Côte, F.-X., & Berthouly, M. (1998). La propagacion masal de los hibridos F1 de cafes arabicos. Boletín de Promecafé, 80, 12‑15.

  • Bertrand, B., Cilas, C., Hervé, G., Anthony, F., Etienne, H., & Villain, L. (1998). Relations entre les populations des nématodes #Meloidogyne exigua# et #Pratylenchus# sp., dans les racines de #Coffea arabica# au Costa Rica. Plantations, Recherche, Développement, 5(4), 279‑286.

  • Etienne, H., Solano, W., Pereira, A., Bertrand, B., & Berthouly, M. (1997). Protocole d’acclimatation de plantules de caféiers produites #in vitro# (CIRAD-CP - FRA; CATIE - CRI; PROMECAFE - CRI; ICAFE - CRI, Trad.). Plantations, Recherche, Développement, 4(5), 304‑311.

  • Etienne, H., Lartaud, M., Michaux-Ferrière, N., Carron, M.-P., Berthouly, M., & Teisson, C. (1997). Improvement of somatic embryogenesis in Hevea brasiliensis (Muell. Arg.) using the temporary immersion technique (CIRAD-GERDAT - FRA; CIRAD-CP - FRA, Trad.). In Vitro Cellular and Developmental Biology. Plant, 33(2), 81‑87.

  • Etienne, H., Lartaud, M., Carron, M.-P., & Michaux-Ferrière, N. (1997). Use of calcium to optimize long-term proliferation of friable embryogenic calluses and plant regeneration in Hevea brasiliensis (Muell. Arg.) (CIRAD-CP - FRA; CATIE - CRI; CIRAD-BIOTROP - FRA, Trad.). Journal of Experimental Botany, 48(306), 129‑137. https://doi.org/10.1093/jxb/48.1.129

  • Engelmann, F., Lartaud, M., Chabrillange, N., Carron, M.-P., & Etienne, H. (1997). Cryopreservation of embryogenic calluses of two commercial clones of Hevea brasiliensis (FAO - ITA; CIRAD-CP - FRA; CATIE - CRI, Trad.). CryoLetters, 18, 107‑116.

  • Teisson, C., Alvard, D., Berthouly, M., Côte, F.-X., Escalant, J.-V., & Etienne, H. (1995). Culture #in vitro# par immersion temporaire : Un nouveau récipient (CIRAD-GERDAT - FRA; CIRAD-CP - FRA; CIRAD-FLHOR - REU, Trad.). Plantations, Recherche, Développement, 2(5), 29‑34.

  • Montoro, P., Etienne, H., & Carron, M.-P. (1995). Relation between nitrogen uptake, amino acid contents, and embryogenic intensity of rubber tree calli (CIRAD-CP - FRA, Trad.). Journal of Plant Nutrition, 18(8), 1693‑1704. https://doi.org/10.1080/01904169509365014

  • Montoro, P., Etienne, H., & Carron, M.-P. (1995). Effect of calcium on callus friability and somatic embryogenesis in Hevea brasiliensis Muell. Arg.  : Relations with callus mineral nutrition, nitrogen metabolism and water parameters (CIRAD-CP - FRA, Trad.). Journal of Experimental Botany, 46(283), 255‑261. https://doi.org/10.1093/jxb/46.2.255

  • Montoro, P., Etienne, H., Michaux-Ferrière, N., & Carron, M.-P. (1993). Callus friability and somatic embryogenesis in Hevea brasiliensis (CIRAD-IRCA - FRA; CIRAD-BIOTROP - FRA, Trad.). Plant Cell, Tissue and Organ Culture, 33(3), 331‑338. https://doi.org/10.1007/BF02319019

  • Etienne, H., Sotta, B., Montoro, P., Miginiac, E., & Carron, M.-P. (1993). Relations between exogenous growth regulators and endogenous indole-3-acetic acid and abscisic acid in the expression of somatic embryogenesis in Hevea brasiliensis (Muell. Arg.) (CIRAD-CP - FRA, Trad.). Plant Science, 88, 91‑96. https://doi.org/10.1016/0168-9452(93)90113-E

  • Etienne, H., Sotta, B., Montoro, P., Miginiac, E., & Carron, M.-P. (1993). Comparison of endogenous ABA and IAA contents in somatic and zygotic embryos of Hevea brasiliensis (Muell. Arg.) during ontogenesis (CIRAD-CP - FRA, Trad.). Plant Science, 92(1), 111‑119. https://doi.org/10.1016/0168-9452(93)90071-7

  • Etienne, H., Montoro, P., Michaux-Ferrière, N., & Carron, M.-P. (1993). Effects of dessication, medium osmolarity and abscicic acid on the maturation of Hevea brasiliensis somatic embryos (CIRAD-CP - FRA; CIRAD-BIOTROP - FRA, Trad.). Journal of Experimental Botany, 44(267), 1613‑1619. https://doi.org/10.1093/jxb/44.10.1613

  • Montoro, P., Etienne, H., & Carron, M.-P. (1992). Embryogenèse somatique entretenue chez Hevea brasiliensis (CIRAD-CP - FRA, Trad.). Revue de Cytologie et de Biologie Végétales, Le Botaniste, 17 Q-Maintanable somatic embryogenesis in Hevea brasiliensis, 113‑119.

  • Carron, M.-P., D’Auzac, J., Etienne, H., El Hadrami, I., Housti, F., Michaux-Ferrière, N., & Montoro, P. (1992). Biochemical and histological features of somatic embryogenesis in Hevea brasiliensis (CIRAD-CP - FRA, Trad.). Indian Journal of Natural Rubber Research, 5(1‑2), 7‑17.

  • Montoro, P., Etienne, H., Carron, M.-P., & Nougarède, A. (1992). Incidence des cytokinines sur l’induction de l’embryogenèse et la qualité des embryons somatiques chez #Hevea brasiliensis# Müll. Arg (CIRAD-IRCA - FRA, Trad.). Comptes Rendus de l’Académie des Sciences. Série 3, Sciences de la Vie, 315, 567‑574.

  • Etienne, H., Montoro, P., & Carron, M.-P. (1991). Incidence des paramètres hydriques sur le développement des cals d’Hevea brasiliensis en culture #in vitro# (CIRAD-IRCA - FRA, Trad.). Annales des Sciences Forestières, 48 Q-The effect of water parameters on the development of Hevea brasiliensis calli in #in vitro# culture, 253‑265. https://doi.org/10.1051/forest:19910302

  • Etienne, H., Berger, A., & Carron, M.-P. (1991). Water status of callus from #Hevea brasiliensis# during induction of somatic embryogenesis (CIRAD-IRCA - FRA; CNRS - FRA, Trad.). Physiologia Plantarum, 82(2), 213‑218. https://doi.org/10.1111/j.1399-3054.1991.tb00083.x

Books and chapters

  • Bertrand, B., Breitler, J.-C., Georget, F., Penot, E., Bordeaux, M., Marraccini, P., Leran, S., Campa, C., Bonato, O., Villain, L., & Etienne, H. (2019). New varieties for innovative agroforestry coffee systems. In P.-M. E. (ed. ) Côte François-Xavier (ed.) Perret Sylvain (ed. ), Roudier Philippe (ed. ), Bruno Rapidel (ed. ), Thirion Marie-Cécile (ed. ). (Éd.), The agroecological transition of agricultural systems in the Global South (Agritrop : 592997; p. 161‑176). Ed. Quae; https://www.quae-open.com/produit/114/9782759230570/the-agroecological-transition-of-agricultural-systems-in-the-global-south

  • Bertrand, B., Breitler, J.-C., Georget, F., Penot, E., Bordeaux, M., Marraccini, P., Leran, S., Campa, C., Bonato, O., Villain, L., & Etienne, H. (2018). De nouvelles variétés pour des systèmes caféiers agroforestiers innovants. In P.-M. E. (ed. ) Côte François-Xavier (ed.) Perret Sylvain (ed. ), Roudier Philippe (ed. ), Bruno Rapidel (ed. ), Thirion Marie-Cécile (ed. ). (Éd.), La transition agro-écologique des agricultures du Sud (Agritrop : 590491; p. 231‑256). Ed. Quae; https://www.quae.com/produit/1546/9782759228232/la-transition-agro-ecologique-des-agricultures-du-sud

  • Etienne, H., Guyot, R., Beulé, T., Breitler, J.-C., & Jaligot, E. (2016). Plant fidelity in somatic embryogenesis-regenerated plants. In O.-A. N. (ed. ) M Loyola-Vargas Victor (ed.) (Éd.), Somatic embryogenesis : Fundamental aspects and applications (Agritrop : 581502; p. 121‑150). Springer International Publishing; https://doi.org/10.1007/978-3-319-33705-0_8

  • Bertrand, B., Marraccini, P., Villain, L., Breitler, J.-C., & Etienne, H. (2016). Healthy tropical plants to mitigate the impact of climate change—As exemplified in coffee. In M. D. (trad. ) Torquebiau Emmanuel (ed.) Cowan Paul (trad. ). (Éd.), Climate change and agriculture worldwide (Agritrop : 578422; p. 83‑95). Springer; https://doi.org/10.1007/978-94-017-7462-8_7

  • Dechamp, E., Breitler, J.-C., Leroy, T., & Etienne, H. (2015). Coffee (#Coffea arabica L.#). In Wang Kan  (ed.) (Éd.), Agrobacterium Protocols : Volume 2 (Agritrop : 577283; 3rd ed, 1‑1224, p. 275‑291). Springer; https://doi.org/10.1007/978-1-4939-1658-0_22

  • Bertrand, B., Marraccini, P., Villain, L., Breitler, J.-C., & Etienne, H. (2015). La bonne santé des plantes tropicales pour atténuer les effets du changement climatique : L’exemple du caféier. In Torquebiau Emmanuel (ed.) (Éd.), Changement climatique et agricultures du monde (Agritrop : 575564; p. 84‑95). Ed. Quae;
  • Anthony, F., Bertrand, B., Etienne, H., & Lashermes, P. (2011). #Coffea# and #Psilanthus#. In Kole Chittaranjan (ed.) (Éd.), Wild crop relatives : Genomic and breeding resources. Plantation and ornamental crops (Agritrop : 561797; p. 41‑61). Springer [Allemagne]; https://doi.org/10.1007/978-3-642-21201-7_3

  • Lashermes, P., Bertrand, B., & Etienne, H. (2009). Breeding coffee (#Coffea arabica#) for sustainable production. In P. P. M. (ed. ) Jain Shri Mohan (ed.) (Éd.), Breeding plantation tree crops : Tropical species (Agritrop : 547768; p. 525‑543). Springer [Etats-Unis]; https://doi.org/10.1007/978-0-387-71201-7_14

  • Lashermes, P., Carvalho Andrade, A., & Etienne, H. (2008). Genomics of coffee, one of the world’s largest traded commodities. In M. R. (ed. ) Moore Paul H. (ed.) (Éd.), Genomics of tropical crop plants (Agritrop : 546271; 1‑1, p. 203‑226). Springer [Etats-Unis]; https://doi.org/10.1007/978-0-387-71219-2_9

  • Etienne, H., Lashermes, P., Menendez-Yuffa, A., De Guglielmo-Croquer, Z., Alpizar, E., & Sreenath, H. L. (2008). Coffee. In H. T. C. (ed. ) Kole Chittaranjan (ed.) (Éd.), Compendium of transgenic crop plants. Transgenic plantation crops, ornamentals and turf grasses (Agritrop : 548114; p. 57‑84). Wiley-Blackwell;

  • Leroy, T., Alpizar, E., Dufour, M., & Etienne, H. (2006). Coffee (#Coffea# sp.). In Wang Kan (ed.) (Éd.), Agrobacterium protocols (Agritrop : 535058; 2nd ed., 1‑344, p. 191‑208). Humana Press;

  • Bertrand, B., Etienne, H., Davrieux, F., & Guyot, B. (2006). Central America and the Caribbean, breeding for quality. In Montagnon Christophe (ed.) (Éd.), Coffee : Terroirs and qualities (Agritrop : 536224; p. 147‑161). Ed. Quae;

  • Etienne, H. (2005). Somatic embryogenesis protocol : Coffee (#Coffea arabica# L. and #C. canephora# P.). In G. P. K. (ed. ) Jain Shri Mohan (ed.) (Éd.), Protocol for somatic embryogenesis in woody plants (Agritrop : 528249; 1‑77, p. 167‑179). Springer;

  • Berthouly, M., & Etienne, H. (2005). Temporary immersion system : A new concept for use liquid medium in mass propagation. In P. W. (ed. ) Hvoslef-Eide Anne Kathrine (ed.) (Éd.), Liquid culture systems for in vitro plant propagation (Agritrop : 533338; p. 165‑195). Springer [Pays-Bas];

  • Etienne, H. (2004). Cultivo in vitro del café : Un nuevo instrumetno para la seleccion y la propagacion rapida de los materiales mejorades. In Café en CIRAD (Agritrop : 531638; p. 2 p.). CIRAD-CP;

  • Etienne, H. (2003). La culture in vitro du caféier : Un nouvel outil pour la sélection et la propagation rapide de matériels d’élite. In Le café au Cirad (Agritrop : 531595; p. 2 p.). CIRAD-CP;

  • Bertrand, B., Etienne, H., Davrieux, F., & Guyot, B. (2003). Amérique centrale et Caraïbe, amélioration variétale et qualité. In Montagnon Christophe (ed.) (Éd.), Cafés : Terroirs et qualités (Agritrop : 512742; p. 129‑143). CIRAD;

  • Bertrand, B., Anzueto, F., Moran, M. X., Eskes, A., & Etienne, H. (2002). Création et diffusion par embryogenèse somatique d’une variété porte-greffe (#Coffea canephora#). In Recherche et caféiculture (Agritrop : 508303; 1‑Mai, p. 95‑107). CIRAD-CP;

  • Engelmann, F., & Etienne, H. (2000). Cryopreservation of embryogenic calli of #Hevea brasiliensis#. In G. P. K. (ed. ) Jain Shri Mohan (ed.) Newton Ronald J. (ed. ). (Éd.), Somatic embryogenesis in woody plants (Agritrop : 476711; 1‑6, p. 729‑746). Kluwer Academic Publishers;

  • Etienne, H., Barry-Etienne, D., Vasquez, N., & Berthouly, M. (1999). Aportes de la biotecnologia al mejoramiento genético del café : El ejemplo de la multiplicacion por embriogénesis somatica de hibridos F1 en America Central. In R. B. (ed. ) Bertrand Benoît (ed.) (Éd.), Desafios de la caficultura en Centroamerica (Agritrop : 391795; p. 457‑493). IICA;

  • Carron, M.-P., Seguin, M., Besse, P., Etienne, H., Lardet, L., Leconte, A., & Nicolas, D. (1997). L’hévéa. In Teisson C. (ed.); CNED; AUPELF; UREF (Éd.), & CIRAD-CP - FRA; CATIE - CRI (Trad.), Biotechnologies végétales. Intégration chez les plantes tropicales. 2 (Agritrop : 388962; 1‑2, p. 31‑48). CNED;

  • Carron, M.-P., Etienne, H., Michaux-Ferrière, N., & Montoro, P. (1995). Somatic embryogenesis in rubber tree (Hevea brasiliensis Muell. Arg.). In Bajaj Y.P.S. (ed.) (Éd.), & CIRAD-CP - FRA (Trad.), Somatic embryogenesis and synthetic seed. 1 (Agritrop : 464045; 1‑vol. 30, p. 353‑369). Springer;

  • Carron, M.-P., Etienne, H., Lardet, L., Campagna, S., Perrin, Y., Leconte, A., & Chaine, C. (1995). Somatic embryogenesis in rubber (Hevea brasiliensis Muell. Arg.). In G. P. (ed. ) Jain S. (ed.) Newton R. (ed. ). (Éd.), & CIRAD-CP - FRA (Trad.), Somatic embryogenesis in woody plants (Agritrop : 387702; p. 117‑136). Kluwer Academic Publishers;

  • Grapin, A., Michaux-Ferrière, N., Etienne, H., & Carron, M.-P. (1994). Hypoxie et embryogenèse somatique : #Hevea brasiliensis#. In Teisson Claude (ed.) (Éd.), & CIRAD-GERDAT-BIOTROP/CIV - FRA; CIRAD-CP-HEVEA - FRA (Trad.), La culture in vitro de plantes tropicales (Agritrop : 387307; p. 23‑25). CIRAD;

  • Etienne, H., Sotta, B., Montoro, P., Miginiac, E., & Carron, M.-P. (1994). Régulateurs de croissance en embryogénèse somatique de l’hévéa. In Teisson Claude (ed.) (Éd.), & CIRAD-GERDAT-BIOTROP/CIV - FRA; CNRS - FRA; CIRAD-CP-HEVEA - FRA (Trad.), La culture in vitro de plantes tropicales (Agritrop : 387308; p. 31‑35). CIRAD;

  • Etienne, H., Montoro, P., & Carron, M.-P. (1991). Les paramètres hydriques en culture #in vitro# : Facteurs limitants de l’embryogénèse somatique chez Hevea brasiliensis. In CIRAD-IRCA - FRA; CIRAD-BIOTROP - FRA (Trad.), Culture in vitro : Illustrations de quelques recherches = [In vitro culture : Rendering of some researchings]. - Montpellier : CIRAD, 1991 (Agritrop : 427787; p. 20‑21). s.n.;


  • Etienne, H., Georget, F., Bertrand, B., & CIRAD-BIOS-UMR RPB - FRA. (2014). Récipient pour la culture in vitro de matériel végétal en conditions stériles, par immersion temporaire (Brevet FR2974580 B1; WO2012/146872 A1; BR112013027846 A2; MX2013012473 A). https://patents.google.com/patent/WO2012146872A1/und

Research of the COFFEADAPT team
COFFEEADAPT team publication
Team members
Permanent and temporary staff